Experimental and numerical investigation into the joule heating effect for electrokinetically driven microfluidic chips utilizing total internal reflection fluorescence microscopy |
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Authors: | Lung-Ming Fu Jing-Hui Wang Wen-Bo Luo Che-Hsin Lin |
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Affiliation: | (1) Department of Materials Engineering, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan;(2) Department of Mechanical and Electro-Mechanical Engineering, National Sun Yat-sen University, Kaohsiung, 804, Taiwan;(3) Advanced Crystal Opto-Electronics Research Center, National Sun Yat-sen University, Kaohsiung, 804, Taiwan;(4) College of Mechanical Engineering, University of Science and Technology Beijing, 10003 Beijing, China |
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Abstract: | This paper presents a detection scheme for analyzing the temperature distribution nearby the channel wall in a microfluidic
chip utilizing a temperature-dependent fluorescence dye. An advanced optical microscope system—total internal reflection fluorescence
microscope (TIRFM) is used for measuring the temperature distribution on the channel wall at the point of electroosmotic flow
in an electrokinetically driven microfluidic chip. In order to meet the short working distance of the objective type TIRFM
scheme, microscope cover glass slits are used to fabricate the microfluidic chips. The short fluorescence excitation depth
from a TIRFM system makes the intensity information obtained using TIRFM is not sensitive to the channel depth variation which
ususally biases the measured results while using a conventional Epi-fluorescence microscope (EPI-FM). Therefore, a TIRFM can
precisely describe the temperature profile of the distance within 100 nm of the channel wall where consists of the Stern layer
and the diffusion layer for an electrokinetic microfluidic system. Results indicate the proposed TIRFM provides higher measurement
sensitivity over the EPI-FM. Significant temperature gradient along the channel depth is experimentally observed. In addition,
the measured wall temperature distributions can be the boundary conditions for numerical investigation into the joule heating
effect. The proposed method gives a precise temperature profile of microfluidic channels and shows the substantial impact
on developing a numerical simulation model for precisely predicting the joule heating effect in microfluidic chips. |
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Keywords: | Joule heating effect Microfluidic chip Total internal reflection fluorescence microscope |
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