| 大孔吸附树脂分离纯化蛹虫草固体培养基中虫草素工艺 |
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| 引用本文： | 胡瑕,谢红旗,罗巍,夏志兰,刘东波.大孔吸附树脂分离纯化蛹虫草固体培养基中虫草素工艺[J].食品科学,2012,33(6):110-114. |
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| 作者姓名： | 胡瑕 谢红旗 罗巍 夏志兰 刘东波 |
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| 作者单位： | 1.湖南农业大学园艺园林学院，国家中医药管理局亚健康干预技术实验室
2.国家柑橘改良中心长沙分中心 |
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| 基金项目： | 长沙市科技计划重点项目(K0902020-11) |
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| 摘 要： | 比较D101、AB-8、HPD-100、HPD-400、HPD-500、HPD-722、DM130七种大孔吸附树脂对蛹虫草固体培养基中虫草素的吸附与解吸性能，筛选出HPD-100树脂为最佳树脂，并确定HPD-100树脂吸附分离最佳工艺条件：上样液质量浓度0.6mg/mL、上样流速3BV/h、上样体积6BV；解吸剂为体积分数25%乙醇溶液、解吸流速2BV/h、解吸体积4BV。根据此工艺条件，蛹虫草固体培养基粗提物经HPD-100树脂纯化后，虫草素产品纯度可达14.1%，较粗提物产品提高了8倍多。
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| 关 键 词： | 虫草素 蛹虫草 大孔吸附树脂 |
Separation and Purification of Cordycepin from Spent Solid Culture Medium after Harvesting Cordyceps militaris Fruit Bodies by Macroporous Resin Adsorption |
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| HU Xia,XIE Hong-qi,LUO Wei,XIA Zhi-lan,LIU Dong-bo.Separation and Purification of Cordycepin from Spent Solid Culture Medium after Harvesting Cordyceps militaris Fruit Bodies by Macroporous Resin Adsorption[J].Food Science,2012,33(6):110-114. |
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| Authors: | HU Xia XIE Hong-qi LUO Wei XIA Zhi-lan LIU Dong-bo |
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| Affiliation: | (1. Sub-health Intervention Technology Laboratory, State Administration of Traditional Chinese Medicine, College of Horticulture and
Landscape, Hunan Agricultural University, Changsha 410128, China；
2. Changsha Subcenter of National Center of Citrus Improvement, Changsha 410128, China ) |
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| Abstract: | The adsorption and desorption characteristics of macroporous resins D101,AB-8,HPD-100,HPD-400,HPD-500,HPD-722 and DM130 for cordycepin from spent solid culture medium after harvesting Cordyceps militaris fruit bodies were comparatively investigated.The results showed that HPD-100 was the best resin for the purification of cordycepin from the spent culture medium.The optimal separation and purification conditions were cordycepin concentration of 0.6 mg/mL,sample loading amount of 6 BV,sample loading flow rate of 3 BV/h,4 BV of 25% ethanol as desorbent,and desorption flow rate of 2 BV/h.Under these conditions,the purity of cordycepin could reach up to 14.1%,which was 8 times higher than before purification. |
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| Keywords: | cordycepin Cordyceps militaris macroporous resin |
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