| 从蛋黄中提取及纯化卵磷脂 |
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| 引用本文： | 许晓慧,赵彬侠,赵立新.从蛋黄中提取及纯化卵磷脂[J].食品科学,2006,27(8):129-132. |
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| 作者姓名： | 许晓慧 赵彬侠 赵立新 |
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| 作者单位： | 山东淄博矿业集团技术中心； 西北大学化工系 |
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| 摘 要： | 本文以鸡蛋的卵黄为原料,采用含水乙醇与乙醚提取,通过丙酮脱水去油,得到粗品卵磷脂;进一步以氯仿/甲醇为洗脱液,硅胶柱层析法分离纯化自制的粗品卵磷脂,得到高纯度的纯白色固体卵磷脂,由薄层色谱定性检测,仅含一个斑点;用薄层扫描仪定量检测纯度大于97.4%。
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| 关 键 词： | 蛋黄 卵磷脂 提取 纯化 层析法 |
| 文章编号： | 1002-6630（2006）08-0129-04 |
| 收稿时间： | 2005-03-31 |
| 修稿时间： | 2005-03-31 |
Extraction and Purification of Phosphatidylcholine from Egg Yolk |
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| XU Xiao-hui,ZHAO Bin-xia,ZHAO Li-xin.Extraction and Purification of Phosphatidylcholine from Egg Yolk[J].Food Science,2006,27(8):129-132. |
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| Authors: | XU Xiao-hui ZHAO Bin-xia ZHAO Li-xin |
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| Affiliation: | 1.The Technology Center of Zibo Mining Group Ltd.,Zibo 255120,China;2.Department of Chemical Engineering,Northwest University,Xi′an 710069,China |
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| Abstract: | With egg yolk as raw material,extracted with aqueous ethanol and ether,and removing water and oil with acetone,a crude phospholipids mixture was obtained.Then the thin-layer chromatography was used with column on silicic acid to purify the phospholipids mixture.Eluated with the solvent mixture(choroform/methanol),ultimately a highly pure white lecithin was obtained.Analysised by thin layer chromatography,there is only one spot.With thin-layer chromatography-scanning method to assay the purity it is higher than 97.4%. |
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| Keywords: | yolk phosphatidylcholine extraction purification chromatography on silicic acid |
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