蔗果低聚糖浆成分的高效液相色谱及核磁共振结构分析

本文利用两种不同分离性能的高效波相色谱系统分别对我们生产的蔗果低聚糖浆产品与国外的同类产品进行了对照分析。结果表明，该糖浆中蔗果低聚精的含量超过55％，主要由蔗果三精（1-kestose）、蔗果四糖（nystose）和蔗果五精（1，1，1－kestonentaose）组成。从糖浆中分离纯化出的各低聚精纯样循一维、二维碳氢核磁共振港进一步确证了各糖成份的化学结构。由此证明，该糖浆产品的组成和各成份的含量均达到了国外同类产品（NeosugarG）的标准。这一研究将对该产品今后进入国内功能性食品添加剂市场和生产质量监控具有重要指导意义。

High Performance Liquid Chromatographic Analysis of Zheguo Fructooligosaccharide Syrup and Nuclear Magnetic Resonance Spectra of Its Components

The fructooligosaccharide syrup, named Zheguo syrup in Chinese, wasproduced enzymatically from sucrose in our laboratory. To ensure the Zheguofructooligosaccharide syrup had the correct constituents and to be confident of the qualitycontrol in its production, chromatographic separation and nuclear magnetic resonance(NMR) analysis were carried out on this syrup. High performance liquid chromatographic (HPLC) analysis showed that the syrup contained more than 55% fructooligosacchrides .the main components of which were identified as 1-kestose, nystose and 1, 1, 1 -kestopentose.1 - D and 2 - D of NMR spectral data obtained from pure samples of these cornponents,isolated from the syrup, verified the identifications of the HPLC peaks- Compared to the commercial Neosugar seriers products, the Zheguo syrup contained glucose, fructose,sucrose, 1 -kestose, nystose and 1, 1, 1 -kestopentose with 30%, 1 %, 10%, 25 %, 29% .5%, respectively,which were similar to those in Neosugar G products. The results from this study will provide important information necessary to obtain premarket approval for the use of Zheguo syrup as an low-calorie food additive into the Chinese market.