Identification of Aminoimidazole and Aminothiazole Derivatives as Src Family Kinase Inhibitors |
| |
Authors: | Dr Cinzia Maria Francini Dr Anna Lucia Fallacara Dr Roberto Artusi Dr Laura Mennuni Dr Alessia Calgani Dr Adriano Angelucci Prof Silvia Schenone Prof Maurizio Botta |
| |
Affiliation: | 1. Medicinal Chemistry Division, Rottapharm Biotech S.r.l., Monza, Italy;2. Dipartimento di Biotecnologie, Chimica e Farmacia, Università degli Studi di Siena, Siena, Italy;3. Pharmacology and Toxicology Division, Rottapharm Biotech S.r.l., Monza, Italy;4. Dipartimento di Scienze Cliniche Applicate e Biotecnologie, Università dell'Aquila, L'Aquila, Italy;5. Dipartimento di Farmacia, Università degli Studi di Genova, Genova, Italy;6. Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, BioLife Science Building, Suite 333, Philadelphia, PA, USA |
| |
Abstract: | Src family kinases (SFKs) are a family of non‐receptor tyrosine kinases (TKs) implicated in the regulation of many cellular processes. The aberrant activity of these TKs has been associated with the growth and progression of cancer. In particular, c‐Src is overexpressed or hyperactivated in a variety of solid tumors and is most likely a strong promoting factor for the development of metastasis. Herein, the synthesis of new 4‐aminoimidazole and 2‐aminothiazole derivatives and their in vitro biological evaluation are described for their potential use as SFK inhibitors. Initially, 2‐aminothiazole analogues of dasatinib and 4‐aminoimidazole derivatives were synthesized and tested against the SFKs Src, Fyn, Lyn, and Yes. Five hits were identified as the most promising compounds, with Ki values in the range of 90–480 nm . A combination of molecular docking, homology modeling, and molecular dynamics were then used to investigate the possible binding mode of such compounds within the ATP binding site of the SFKs. Finally, the antiproliferative activities of the best candidates were evaluated against SH‐SY5Y and K562 cell lines. Compound 3 b 2‐(4‐{2‐methyl‐6‐(5‐phenylthiazol‐2‐yl)amino]pyrimidin‐4‐yl}piperazin‐1‐yl)ethanol] was found to be the most active inhibitor. |
| |
Keywords: | aminoimidazoles aminothiazoles antitumor agents inhibitors Src kinase |
|
|