Different human tenascin-C variants in the extracellular matrix of cultured human fibroblasts |
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Authors: | A Siri G Allemanni B Gaggero L Zardi |
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Affiliation: | Department of Radiology Hospital, University of Pennsylvania, Philadelphia 19104, USA. |
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Abstract: | Using an immunoadsorbent prepared with a monoclonal antibody specific for the high molecular mass isoform of human tenascin-C, we purified tenascin-C molecules containing at least one large subunit from the extracellular matrix of cultured normal human fibroblasts. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting analyses have shown that both high and low molecular mass subunits are present in these tenascin-C preparations. Because the monoclonal antibody used is able to bind only the high molecular mass isoform, the present data show that part of the tenascin-C present in the fibroblast extracellular matrix is made up of heterohexameric molecules. |
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