Direct analysis of trivernolin by high-performance liquid chromatography

A rapid liquid chromatographic method for the quantitation of trivernolin in epoxy seed oils has been developed. A weighed sample of the oil is dissolved in chloroform (～100–300 mg/ml), and a 1–20µl portion of the oil is chromatographed on a 2 ft×1/4 in. OD C₁₈ µ-Bondapak column with acetonitrile-acetone (2:1, v/v). The area of the trivernolin peak from the differential refractometer recorder tracing is converted toµg trivernolin by use of a calibration curve of peak area vs. trivernolin weight.