SEM images of DNA double helix and nucleosomes observed by ultrahigh-resolution scanning electron microscopy. |
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Authors: | S Inaga H Osatake K Tanaka |
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Affiliation: | Department of Anatomy, Faculty of Medicine, Tottori University, Yonago, Japan. |
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Abstract: | We observed DNA double helix and nucleosomes in the chromatin of chicken erythrocytes by ultrahigh-resolution scanning electron microscopy. Specimens were prepared according to a modified microspreading technique in combination with the carbon plate method and observed without metal coating. A part of the DNA fibers without nucleosomes showed "left-handed" double-strands with twisting appearances and regular periodicities of the helix. Linker DNA between the nucleosomes showed "right-handed" appearances. Most of the nucleosome particles appeared as prolate ellipsoidal shapes of various sizes. DNA appeared to enter and exit the nucleosome particles on opposite sides winding around the histone core. |
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