Spectrofluorimetric,spectrophotometric and chemometric analysis of wheat grains infested by Sitophilus granarius |
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| Affiliation: | 1. Department of Chemistry, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8/A, 31000 Osijek, Croatia;2. Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, SI-1000 Ljubljana, Slovenia;3. Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Croatia;2. Department of Pharmaceutical Sciences, University of Maryland Eastern Shore School of Pharmacy, Princess Anne, MD, United States;3. University of Maryland Eastern Shore School of Pharmacy, Princess Anne, MD, United States;1. Department of Chemistry, J. J. Strossmayer University, HR-31000 Osijek, Croatia;2. Department of Biotechnology, University of Rijeka, HR-51000 Rijeka, Croatia;3. Faculty of Food Technology, J. J. Strssmayer University, HR-31000 Osijek, Croatia;4. Center for Proteomics, Medical School, University of Rijeka, HR-51000 Rijeka, Croatia;5. Department of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Providence, RI, USA;6. Department of Medicine, Warren Alpert Medical School, Brown University, Providence, RI, USA |
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| Abstract: | Invisible infestation of wheat grains by Sitophilus granarius during postharvest handling is one of the main problems particularly for small enterprises and farm grain storage facilities due to the lack of cheap and fast methods for early detection of infestation, especially when only a small proportion of grain is infested by larvae. In order to determine whether the spectrofluorimetric, spectrophotometric or chemometric analysis of various extracts of wheat grains infested by S. granarius larvae could be used for detection of infested grain, four different types of extracts: 50 mmol dm−3 Tris–HCl pH 8.9, aqueous iso-propanol (φ = 0.55), methanol, and aqueous trichloroacetic acid (γ = 0.2 mg cm−3), of non-infested and infested wheat grains have been examined for fluorescence properties and UV/VIS spectral characteristics, while the chemometric analysis included determination of protein, reactive amino groups, polyphenols and soluble sugar content. Analysis of extracts revealed significant differences in UV/VIS spectral characteristics, level of reactive amino groups and polyphenol content between infested and non-infested grains. The results obtained indicate that instrumental analysis of spectral characteristics of grain extracts could be used as a method for determination of grain infestation. |
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