Tissue Engineering of Cartilage on Ground-Based Facilities |
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Authors: | Ganna Aleshcheva Johann Bauer Ruth Hemmersbach Marcel Egli Markus Wehland Daniela Grimm |
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Affiliation: | 1.Clinic for Plastic, Aesthetic and Hand Surgery,Otto-von-Guericke University,Magdeburg,Germany;2.Max-Planck-Institute for Biochemistry,Martinsried,Germany;3.German Aerospace Centre (DLR), Gravitational Biology,Cologne,Germany;4.Aerospace Biomedical Science & Technology Space Biology Group,Lucerne University of Applied Sciences and Arts,Hergiswil,Switzerland;5.Department of Biomedicine,Aarhus University,Aarhus C,Denmark |
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Abstract: | Investigations under simulated microgravity offer the opportunity for a better understanding of the influence of altered gravity on cells and the scaffold-free three-dimensional (3D) tissue formation. To investigate the short-term influence, human chondrocytes were cultivated for 2 h, 4 h, 16 h, and 24 h on a 2D Fast-Rotating Clinostat (FRC) in DMEM/F-12 medium supplemented with 10 % FCS. We detected holes in the vimentin network, perinuclear accumulations of vimentin after 2 h, and changes in the chondrocytes shape visualised by F-actin staining after 4 h of FRC-exposure. Scaffold-free cultivation of chondrocytes for 7 d on the Random Positioning Machine (RPM), the FRC and the Rotating Wall Vessel (RWV) resulted in spheroid formation, a phenomenon already known from spaceflight experiments with chondrocytes (MIR Space Station) and thyroid cancer cells (SimBox/Shenzhou-8 space mission). The experiments enabled by the ESA-CORA-GBF programme gave us an optimal opportunity to study gravity-related cellular processes, validate ground-based facilities for our chosen cell system, and prepare long-term experiments under real microgravity conditions in space |
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