| 猪链球菌2型的分离与鉴定 |
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| 引用本文： | 高巍,韩文瑜,雷连成,高尚庆,杜崇涛. 猪链球菌2型的分离与鉴定[J]. 中国生物制品学杂志, 2007, 20(6): 447-449,456 |
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| 作者姓名： | 高巍 韩文瑜 雷连成 高尚庆 杜崇涛 |
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| 作者单位： | 吉林大学畜牧兽医学院预防兽医系 (长春130062) |
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| 摘 要： | 目的对从四川省某猪场分离到的5株细菌进行系统鉴定。方法观察菌体的形态、培养特性、动物致病性和生化反应,并采用PCR法鉴定其血清型和毒力因子。结果5株细菌均为猪链球菌2型,均有毒力因子MRP和EF,其中S1株、S2株和S5株对BALB/c小鼠有致病性,另2株无致病性。结论该猪场的疫情是由猪链球菌2型引起的。本研究为进一步预防和控制猪链球菌2型的流行提供了依据。
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| 关 键 词： | 猪链球菌2型 分离 鉴定 |
| 文章编号： | 1004-5503（2007）06-447-04 |
| 收稿时间： | 2006-11-08 |
| 修稿时间： | 2006-11-08 |
Isolation and Identification of Swine Streptococus suis Serotype 2 |
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| GAO Wei, HAN Wen-yu, LEI Lian-cheng, et al. Isolation and Identification of Swine Streptococus suis Serotype 2[J]. Chinese Journal of Bilogicals, 2007, 20(6): 447-449,456 |
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| Authors: | GAO Wei HAN Wen-yu LEI Lian-cheng et al |
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| Affiliation: | College of Animal Science and Veterinary Medicine,Jilin University, Changchun 130062, China |
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| Abstract: | Objective To identify the 5 strains of swine Streptococus suis isolated from a pig farm in Sichuan Province,China.Methods Observe the morphology,culture characteristics,pathogenicity in animals and biochemical reactions of the 5 strains of swine Streptococus suis,and identify their serotypes and virulence factors by PCR.Results All the 5 strains were identified as swine Streptococus suis serotype 2 and contained virulence factors MRP and EF,of which strains S1,S2 and S5 showed pathogenicity in BALB/c mice while the other 2 strains showed no pathogenicity.Conclusion The pestilence in the pig farm was caused by swine Streptococus suis serotype 2,which provided a basis for further prevention and control of infection with swine Streptococus suis serotype 2. |
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| Keywords: | Swine Streptococus suis serotype 2 Isolation Identification |
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