Real-time in vivo imaging collagen in lymphedematous skin using multiphoton microscopy |
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Authors: | Wu Xiufeng Zhuo Shuangmu Chen Jianxin Liu Ningfei |
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Affiliation: | Lymphology Center of Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China. |
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Abstract: | Changes of dermal collagen are characteristic for chronic lymphedema. To evaluate these changes, a real-time imaging based on two-photon excited fluorescence and second-harmonic generation was developed for investigating collagen of lymphedematous mouse and rat tail skin in vivo. Our findings showed that the technique could image the morphological changes and distribution of collagen in lymphedematous mouse and rat tail skin in vivo. More importantly, it may allow visualization of dynamic collagen alteration during the progression of lymphedema. Our findings demonstrated that multiphoton microscopy may have potential in a clinical setting as an in vivo diagnostic and monitoring system for therapy in lymphology. |
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Keywords: | multiphoton microscopy (MPM) second harmonic generation (SHG) image lymphedema collagen |
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