Extraction of bioactive enriched fractions from Eruca sativa leaves by supercritical CO2 technology using different co-solvents |
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| Affiliation: | 1. Department of Industrial Engineering DII, University of Padova, Via Marzolo 9, 35131 Padua, Italy;2. Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Via Marzolo 5, 35131 Padua, Italy;1. Department of Chemistry, University of the Balearic Islands, Ctra Valldemossa km 7.5, 07122 Palma de Mallorca, Spain;2. ASPA Group, Food Technology Department, Polytechnic University of Valencia, Cno Vera s/n, 46021 Valencia, Spain;1. Food Engineering Department, Pamukkale University, 20070 Denizli, Turkey;2. Food Engineering Department, Middle East Technical University, 06531 Ankara, Turkey;1. Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro, Namgu, Busan, 608-737, Republic of Korea;2. Department of Chemical Engineering, Pukyong National University, 365 Sinseon-ro, Namgu, Busan, 608-737, Republic of Korea;3. School of Chemical and Bioengineering, Addis Ababa Institute of Technology, Addis Ababa University, P.O. Box 385, Addis Ababa, Ethiopia;1. Department of Industrial Engineering DII, University of Padua, Via Marzolo 9, 35131 Padua, Italy;2. Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Via Marzolo 5, 35131 Padua, Italy |
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| Abstract: | Supercritical fluid extraction from freeze-dried Eruca sativa leaves is assessed with the aim of studying the feasibility to obtain bioactive enriched fractions containing different classes of valuable compounds. Total extraction yields and compositions using pure CO2 and CO2 + selected co-solvents are compared. Overall extraction curves, fitted by the model of broken and intact cells developed by Sovová, are reported and the influence of the main parameters that affect the extraction process is analysed. The extract with the highest content in glucosinolates and phenols was collected at 30 MPa and 75 °C using 8% (w/w) of water with respect to the CO2 flow rate, whereas the fraction richest in lipids was obtained using 8% (w/w) of ethanol as co-solvent at 45 °C and 30 MPa. A process including a first step with supercritical CO2 extraction using water as co-solvent followed by a second step, where a fraction rich in lipids is extracted using ethanol as co-solvent, is proposed. SCCO2 results are compared with Soxhlet and other methods that combine organic solvents with ultrasounds. |
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| Keywords: | Supercritical extraction Co-solvents Glucosinolates Phenols Lipids |
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