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Gold nanoparticle-mediated fluorescence enhancement by two-photon polymerized 3D microstructures
Affiliation:1. Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged 6726, Hungary;2. University of Szeged, Department of Mineralogy, Geochemistry and Petrology, Egyetem Street 2, Szeged 6722, Hungary;1. Service of Immunology, Hospital Clínic de Barcelona, Barcelona, Spain;2. AIDS Research Group, and Catalonian Center for HIV Vaccines (HIVACAT), Barcelona, Spain;3. Institut de Biology et Chimie des Protéines, UMR5305, UCBL, 7 Passage du Vercors, 69367 Lyon Cedex 07, France;4. Department of Microbiology and Parasitology, Pharmacy Faculty, Universitat de Barcelona, Barcelona, Spain;5. Infectious Diseases and AIDS Unit, Hospital Clínic de Barcelona, Barcelona, Spain;6. Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Barcelona, Barcelona, Spain;1. Departamento de Física, Ingeniería de Sistemas y Teoría de la Señal, Universidad de Alicante, Apartado 99, E-03080 Alicante, Spain;2. Instituto Universitario de Física Aplicada a las Ciencias y las Tecnologías, Universidad de Alicante, Apartado 99, E-03080 Alicante, Spain;3. Departamento de Óptica, Farmacología y Anatomía, Universidad de Alicante, Apartado 99, E-03080 Alicante, Spain;1. Institute of Technical Physics and Materials Science, Research Centre for Natural Sciences, H-1525 Budapest, PO Box 49, Hungary, http://www.nanotechnology.hu/;2. Hungarian Natural History Museum, Baross utca 13, H-1088 Budapest, Hungary;1. Institute of Physics, Nicolaus Copernicus University, 5/7 Grudziądzka Str., 87-100 Toruń, Poland;2. Vlokh Institute of Physical Optics, Sector of Spectroscopy, 23 Dragomarov Str., 79-005 Lviv, Ukraine;3. Institute of Physics, University of Zielona Góra, 4a Szafrana Str., 65-069 Zielona Góra, Poland;1. Experimental Physics Department, Ural Federal University, 19 Mira Street, Yekaterinburg 620002, Russia;2. Institute of Physics, University of Tartu, 14c Ravila Street, Tartu 50411, Estonia;1. Applied Science College, Harbin University of Science and Technology, Harbin 150080, China;2. Department of Chemistry, Harbin Institute of Technology, Harbin 150001, China
Abstract:Fluorescence enhancement achieved by functionalized microstructures made by two-photon polymerization (TPP) is reported for the first time. Microstructures of various shapes made of SU-8 photoresist were prepared and coated with gold nanoparticles (NP) of 80 nm. Localized fluorescence enhancement was demonstrated by microstructures equipped with tips of sub-micron dimensions. The enhancement was realized by positioning the NP-coated structures over fluorescent protein layers. Two fluorophores with their absorption in the red and in the green region of the VIS spectrum were used. Laser scanning confocal microscopy was used to quantify the enhancement. The enhancement factor was as high as 6 in areas of several square-micrometers and more than 3 in the case of local enhancement, comparable with literature values for similar nanoparticles. The structured pattern of the observed fluorescence intensity indicates a classic enhancement mechanism realized by standing waves over reflecting surfaces. With further development mobile microtools made by TPP and functionalized by metal NPs can be actuated by optical tweezers and position to any fluorescent micro-object, such as single cells to realize localized, targeted fluorescence enhancement.
Keywords:Two-photon polymerization  Microstructure fabrication  Gold nanoparticle  Nanomaterials  Surface functionalization  Fluorescence enhancement
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