Preparation and characteristics of bombyx mori sericin / tussah silk fibroin / gelatin-based blended antibacterial films北大核心CSCD

A large number of wound dressings are currently used in the treatment of burns chronic ulcers etc. An ideal wound dressing should prevent dehydration of the wound and retain a favorable moist environment at the wound interface. It should have good antibacterial properties and be easily removed without trauma too. Wound dressings are generally made of readily available bio-materials that require minimal processing possess nontoxic non-allergenic and antimicrobial properties as well as promote wound healing. Clinically collagen chitin chitosan etc. are usually used as materials for producing wound dressings as these materials are capable of accelerating the healing processes at molecular cellular and individual levels. Tussah silk fibroin bombyx mori sericin and gelatin non-toxic and extensive have good bio-compatibility degradability and film-forming properties which are conducive to cell adhesion. In order to prepare safe biofilms with excellent antibacterial properties we prepared a kind of bombyx mori sericin / tussah silk fibroin / gelatin BS-TSF-GEL blended film by using tussah silk fibroin bombyx mori sericin and gelatin as bio-based materials and polyethylene glycol 200 as the cross-linking agent. Firstly we designed the single factor experiment and orthogonal experiment to optimize the preparation process of the BS-TF-GEL blended film. On this basis the blended film was modified with ethanol of different concentrations to improve its strength and crystallinity. The structure and properties of the blended film were characterized by measuring the dissolution loss rate tensile properties microscopic morphology and crystal structure. Finally antimicrobial peptides were loaded by adsorption to obtain the BS-TSF-GEL antimicrobial film. The results showed that the ratio of the optimum formula for the (BS-TSF-GEL) blended film required a mixture of bombyx mori sericin (4. 50 μg / mL) and tussah silk fibroin (27. 5 mg / mL) with a volume ratio of 2:1 polyethylene glycol 200 and gelatin solution (360 mg / mL) which accounted for 35% 40% and 25% respectively of the total system. The dissolution loss rate of the BS-TSF-GEL blended film was 34. 62% the elongation at break was 34. 15% and the breaking strength was 578. 8 kPa after being treated with anhydrous ethanol. FTIR and X-ray diffraction results showed that Silk Ⅱ dominated the BS-TSF-GEL blended film while Silk Ⅰ was in a small amount. The BS-TSF-GEL blended film was pale yellow and porous on the surface which was the ideal material for loading antimicrobial peptides. The BS-TSF-GEL blended film loaded with antimicrobial peptides had a good inhibitory effect on both Escherichia coli and Staphylococcus aureus. It is expected to be used in the field of disposable biomedical materials such as wound dressings. In this study three natural materials with unique advantages were selected as substrates for biofilms and polyethylene glycol with high bio-safety was used as the cross-linking agent to enhance the application value of the blended film by loading antimicrobial peptides. The results broaden the application field of silkworm silk and provide a new way for the development and exploitation of silk fibroin in biological dressings and other medical bio-materials. Bombyx mori sericin tussah silk fibroin and gelatin have been widely used in biological tissue engineering as natural biological materials. In this study three natural materials were used as the biofilm substrate to successfully prepare excellent-performance BS-TSF-GEL blend film using polyethylene glycol cross-linking and ethanol post-treatment techniques. Moreover specific properties can be conferred by replacing different loading substances such as antimicrobial biofilm carrying antimicrobial peptides and biofilm sustained release carrier loading drugs. In addition 3D printing electrostatic spinning and other technologies can also be used to shape the BS-TSF-GEL into different shapes to meet the application requirements under different conditions. In conclusion the research results explore a new way for the application of silk protein. © 2022 Authors. All rights reserved.