Optimization of subcritical water extraction of antioxidants from Coriandrum sativum seeds by response surface methodology |
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| Affiliation: | 1. Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia;2. CEBB d.o.o. Center for Energy, Biomass and Biotechnology, Matka Laginje 1, 47000 Karlovac, Croatia;1. Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia;2. Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia;3. Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia;1. Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia;2. CEBB d.o.o. Center for Energy, Biomass and Biotechnology, Matka Laginje 1, 47000 Karlovac, Croatia;1. Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia;2. REQUIMTE/LAQV, Instituto Superior de Engenharia do Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida, 431, 4249-015 Porto, Portugal;3. Department of Food Technology, Faculty of Agronomy, University of Kragujevac, Cara Du?ana 34, 32000 ?a?ak, Serbia;4. Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia;1. Institute of Food Science and Biotechnology, University of Hohenheim, Garbenstrasse 25, D-70599 Stuttgart, Germany;2. Department of Food Engineering, Faculty of Engineering, Yeditepe University, 26 A?ustos Yerle?imi, Kay??da?? Cad., 34755 Istanbul, Turkey;3. Biological Science Department, King Abdulaziz University, P. O. Box 80257, Jeddah 21589, Saudi Arabia |
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| Abstract: | Subcritical water extraction (SWE) of antioxidants from Coriandrum sativum seeds (CSS) was optimized by simultaneous maximization of the total phenolics (TP) and total flavonoids (TF) yield and antioxidant activity, using IC50 value. Box–Behnken experimental design (BBD) on three levels and three variables was used for optimization together with response surface methodology (RSM). Influence of temperature (100–200 °C), pressure (30–90 bar) and extraction time (10–30 min) on each response was investigated. Experimentally obtained values were fitted to a second-order polynomial model and multiple regression. Analysis of variance (ANOVA) was used to evaluate model fitness and determine optimal conditions. Moreover, three-dimensional surface plots were generated from employed mathematical model. The optimal SWE conditions obtained in simultaneous optimization were temperature of 200 °C, pressure of 30 bar and extraction time of 28.3 min, while obtained values of TP and TF yields and IC50 value at this experimental point would be 2.5452 g GAE/100 g CSS, 0.6311 g CE/100 g CSS and 0.01372 mg/ml, respectively. Moreover, good and moderate linear correlation was observed between antioxidant activity (IC50 value) and total phenolics content (R2 = 0.965), and total flavonoids content (R2 = 0.709) which indicated that these groups of compounds are responsible for antioxidant activity of C. sativum extracts. |
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| Keywords: | Subcritical water extraction Phenolics Flavonoids Response surface methodology |
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