Cultured rat and purified human Pneumocystis carinii stimulate intra- but not extracellular free radical production in human neutrophils |
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Authors: | T Jensen EM Aliouat B Lundgren OP Settnes A Karlsson C Dahlgren E Dei-Cas N Obel |
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Affiliation: | Department of Infectious Diseases, Rigshospitalet, University of Copenhagen, Denmark. |
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Abstract: | The production of free radicals in human neutrophils was studied in both Pneumocystis carinii derived from cultures of L2 rat lung epithelial-like cells and Pneumocystis carinii purified from human lung. Using the cytochrome C technique, which selectively measured extracellular superoxide generation, hardly any free radical production was observed after stimulation with cultured rat-derived P. carinii. A chemiluminescence technique, which separately measured intra- and extracellular free radical production, was subsequently employed to differentiate the free radical generation. It was established that 1) P. carinii stimulated intra- but not extracellular free radical production in human neutrophils, 2) opsonized cultured rat-derived P. carinii stimulated human neutrophils to a strong intracellular response of superoxide production, and 3) opsonized P. carinii, purified from human lung also stimulated human neutrophils to produce intracellular free radicals. |
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