QuEChERS-超高效液相色谱-串联质谱法同时检测辣椒中5种杀菌剂残留

目的 建立 QuEChERS-超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)同时检测辣椒中多菌灵、甲基硫菌灵、烯酰吗啉、嘧菌酯和苯醚甲环唑5种杀菌剂的分析方法.方法 样品经2.0%乙酸...

Simultaneous determination of 5 kinds of fungicides residues in pepper by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry

A QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method To was established a QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of 5 kinds of fungicides, namely, thiophanate methyl, dimethomorph, azoxystrobin and difenoconazole, in fresh pepper. Methods The samples were separated by extraction extracted with 2.0% acetic acid acetonitrile, purified by 50 mg C18 and 20 mg GCB, and detected by UPLC-MS/MS with matrix external standard method for quantification. Results The linear relationships between the peak areas and the corresponding mass concentrations of the 5 kinds of fungicides were good within the mass concentration range of 0.01?1.00 mg/L, and the correlation coefficients were all greater than 0.989. The average recoveries of the 5 kinds of fungicides at the 0.01, 0.1, and 1.0 mg/kg addition levels were 74%?105%, the relative standard deviations were 2.07%?9.61% (n=5), and the limits of quantification were 0.01 mg/kg. The residual amount of the five fungicides in Guizhou pepper was between <0.01 mg/kg to 0.092 mg/kg. Conclusion This method meets the requirements of pesticide residue detection and analysis, and is suitable for accurate qualitative and quantitative analysis of 5 kinds of pesticides in fresh pepper, market supervision and spot check, etc. The actual samples showed that the application of 5 kinds of fungicides in Guizhou pepper was safe, which did not produce related dietary risks and hazards, and did not affect export trade.