| 米曲霉果糖基转移酶催化合成蔗糖-6-乙酸酯 |
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| 引用本文： | 毛多斌,刘国明,韩亚伟,杨雪鹏,陈利平.米曲霉果糖基转移酶催化合成蔗糖-6-乙酸酯[J].精细化工,2010,27(3). |
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| 作者姓名： | 毛多斌 刘国明 韩亚伟 杨雪鹏 陈利平 |
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| 作者单位： | 郑州轻工业学院,食品与生物工程学院,河南,郑州,450002 |
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| 基金项目： | 国家自然科学基金资助项目(20676127)~~ |
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| 摘 要： | 对来自于米曲霉(Aspergillus oryzae)的果糖基转移酶催化蔗糖(s)与葡萄糖-6-乙酸酯(g-6-a)生成蔗糖-6-乙酸酯(s-6-a)的反应进行了研究。考察了底物质量比m(g-6-a)∶m(s)、反应温度、反应时间、pH、酶的质量浓度对反应的影响。单因素实验结果表明,最优的催化反应条件为:反应温度50℃、pH=6.2、m(g-6-a)∶m(s)=1∶3、反应时间48 h、酶的质量浓度为4.0 g/L。在该条件下,g-6-a的摩尔转化率可达26.69%。
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| 关 键 词： | 米曲霉 蔗糖-6-乙酸酯 果糖基转移酶 生物工程 |
Synthesis of Sucrose-6-acetate Catalyzed by Fructosyltransferase from Aspergillus oryzae |
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| MAO Duo-bin,LIU Guo-ming,HAN Ya-wei,YANG Xue-peng,CHEN Li-ping.Synthesis of Sucrose-6-acetate Catalyzed by Fructosyltransferase from Aspergillus oryzae[J].Fine Chemicals,2010,27(3). |
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| Authors: | MAO Duo-bin LIU Guo-ming HAN Ya-wei YANG Xue-peng CHEN Li-ping |
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| Affiliation: | Zhengzhou University of Light Industry/a>;School of Food and Biological Engineering/a>;Zhengzhou 450002/a>;Henan/a>;China |
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| Abstract: | The synthesis reaction of sucrose-6-acetate(s-6-a)with sucrose(s)and glucose-6-acetate(g-6-a) catalyzed by fructosyltransferase from Aspergillus oryzae was investigated.The effects of mass ratio of reactants m(g-6-a):m(s),reaction temperature,reaction time,pH,mass concentration of enzyme on the reaction were examined.The one-factor experimental results showed that the optimum yield was obtained at 50 ℃,pH=6.2,m(g-6-a):m(s)=1:3,reaction time 48 h and mass concentration of enzyme 4.0 g/L.On this condition the molar convert ratio of g-6-a was 26.69%. |
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| Keywords: | Aspergillus oryzae sucrose-6-acetate fructosyltransferase biological engineering |
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