Effects of sex on formation and properties of plasma very low density lipoprotein in vivo |
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Authors: | Carlos Soler-Argilaga Abraham Danon Henry G Wilcox Murray Heimberg |
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Affiliation: | (1) Present address: Department of Pharmacology, School of Medicine, University of Missouri, 65201 Columbia, Missouri;(2) Department of Pharmacology, School of Medicine, Vanderbilt University, 37332 Nashville, Tennessee;(3) Present address: Clinical Pharmacology Unit, Ben-Gurion University, Center for Health Sciences, Beer-Sheva, Israel |
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Abstract: | The concentration and composition of the very low density lipoprotein (VLDL) lipids and the behavior of the VLDL in a density
gradient in the zonal ultracentrifuge were examined in plasma obtained from normal fed male and female rats before and after
intravenous injection of Triton WR-1339. Concentration of lipids in plasma VLDL of female rats was about half that of male
animals. Following injection with Triton WR-1339, the concentration of VLDL lipids was higher in female rats (triacylglycerol)
or similar (phospholipid, cholesterol, and cholesteryl esters) in both sexes. Female rats secreted much more VLDL triacylglycerol
into the plasma compartment than did the male animals under the same experimental conditions. No differences were observed
in lipid composition of the VLDL or in the position of the VLDL in the zonal rotor after ultracentrifugation in a density
gradient of the lipoprotein from plasma of normal male and female rats before treatment with the detergent. However, after
treatment with Triton, a higher proportion of the VLDL particles isolated from plasma of female rats displayed a more rapid
rate-zonal flotation in the ultracentrifuge than did the VLDL produced by the male. The VLDL secreted by female rats contained
fewer moles of phospholipid and free sterol per mol triacylglycerol than did the VLDL secreted by male animals under identical
experimental conditions. The molar ratio of free cholesterol: cholesteryl ester in the VLDL secreted after treatment with
Triton increased in both male and female rats. Simultaneously, the content of arachidonic acid in phospholipid of VLDL increased
with a concomitant decrease in cholesteryl ester. These changes in fatty acid composition suggest that the inhibitory effect
of Triton on lecithin-cholesterol acyl transferase activity affects the exchange of lipids between VLDL and high density lipoprotein.
It can be concluded from the data reported here that sex influences the concentration of plasma lipids in vivo and the output
and properties of the VLDL.
Presented in part at the 59th annual meeting of the Federation of American Societies for Experimental Biology, Atlantic City,
NJ, April 1975 (1).
Recipient of a Career Development Award from the U.S. Public Health Service, No. 1-K4-HL-70329. |
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