| Abstract: | Various crude starches were hydrolyzed by the synergistic action of α-amylase and glucoamylase in PEG-dextran and PEG-substrate aqueous two phase systems. The hydrolysis products were determined, at different temperatures, by the chromatometric method of phenol-sulfuric acid and by HPLC. It was found that even the use of aqueous biphasic systems presents in general advantages in terms of separation of the products in one phase, PEG-substrate system can be used successfully in the cases of corn and rice starches. The main advantage in the use of starch as one of the polymer needed for the formation of two phases, is that the substitution of the dextran polymer decreases remarkably the cost of the reaction. Prior gelatinization of the starch used, gives higher yields of glucose than in the case of non gelatinized starch and the separation of the phases is satisfactory. |
