| 响应面试验优化Lipozyme TLIM催化制备共轭亚油酸结构脂工艺 |
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| 引用本文： | 王晓雅,刘文强,朱雯婷,增 凯,朱雪梅,熊 华,胡蒋宁. 响应面试验优化Lipozyme TLIM催化制备共轭亚油酸结构脂工艺[J]. 食品科学, 2017, 38(2): 214-219. DOI: 10.7506/spkx1002-6630-201702034 |
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| 作者姓名： | 王晓雅 刘文强 朱雯婷 增 凯 朱雪梅 熊 华 胡蒋宁 |
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| 作者单位： | 1.南昌大学食品学院，食品科学与技术国家重点实验室，江西 南昌 330047； 2.南昌市食品质量卫生安全监督检验中心，江西 南昌 330012 |
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| 基金项目： | 国家自然科学基金地区科学基金项目（31460427）；国家自然科学基金面上项目（31571870）；食品科学与技术国家重点实验室自由探索资助课题（SKLF-ZZB-201510） |
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| 摘 要： | 以Lipozyme TLIM催化茶油和共轭亚油酸乙酯制备共轭亚油酸(conjugated linoleic acid,CLA)结构脂,并调控酰基迁移反应以制备Sn-2位含CLA的结构脂。通过响应面法研究反应温度、水分活度、反应时间和底物物质的量比对总CLA键入量和酰基迁移的影响,并优化反应条件。本实验得到的结构脂中含有36.97%的总CLA,其中12.54%的CLA分布在Sn-2位,所得总CLA及Sn-2位的CLA键入量均高于文献报道。
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| 关 键 词： | Lipozyme TLIM CLA 结构脂 酰基迁移 |
Optimization of Production of Structured Lipids Containing Conjugated Linoleic Acid by Lipozyme TLIM Catalysis |
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| WANG Xiaoya,LIU Wenqiang,ZHU Wenting,ZENG Kai,ZHU Xuemei,XIONG Hua,HU Jiangning. Optimization of Production of Structured Lipids Containing Conjugated Linoleic Acid by Lipozyme TLIM Catalysis[J]. Food Science, 2017, 38(2): 214-219. DOI: 10.7506/spkx1002-6630-201702034 |
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| Authors: | WANG Xiaoya LIU Wenqiang ZHU Wenting ZENG Kai ZHU Xuemei XIONG Hua HU Jiangning |
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| Affiliation: | 1. State Key Laboratory of Food Science and Technology, School of Food Scicence and Technology, Nanchang University, Nanchang 330047, China; 2. Nanchang Food Quality Health Safety Supervision and Inspection Center, Nanchang 330012, China |
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| Abstract: | In this study, structured lipids containing conjugated linoleic acid (CLA) were produced from the reaction between camellia seed oil and CLA ethyl ester catalyzed by Lipozyme TLIM, where Sn-2 positional CLA was obtained by enhancing acyl migration. Four reaction parameters, namely temperature, water activity, reaction time and molar ratio of substrates, were optimized by response surface methodology (RSM) for improved total CLA incorporation and acyl migration. In the synthesized structured lipids, total and Sn-2 positional CLA were up to 36.97% and 12.54% when acyl migration was maximized. The incorporation ratios of total CLA and Sn-2 positional CLA were both higher than the published data. |
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| Keywords: | Lipozyme TLIM CLA structured lipid acyl migration |
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