气相色谱-质谱联用法检测乙烯-乙烯醇活性食品包装膜中的香叶醇和α-松油醇

建立乙烯-乙烯醇包装膜中活性物质香叶醇和α-松油醇的气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)联用检测方法。实验首先讨论了样品前处理条件和GC-MS测定条件,对迁移至模拟液中的活性物质采用所建方法检测,外标法定量分析。结果表明香叶醇和α-松油醇在0.02~2.0 mg/L范围内均线性关系良好,相关系数分别为0.999 2和0.999 0。该方法的检出限分别为0.003 mg/kg和0.007 mg/kg,定量限分别为0.010 mg/kg和0.020 mg/kg。以0.1、0.2 mg/kg和1.0 mg/kg添加水平进行方法学验证,回收率为82.5%~98.5%,相对标准偏差为3.6%~10.4%。本方法快速、简便、灵敏度高,可为进一步研究活性物质在食品包装材料中的释放机理及活性食品包装膜的应用安全性方面提供有效的检测方法。

Gas Chromatography-Mass Spectrometry Method for Rapid Detection of Geraniol and α-Terpilenol in Ethylene-Vinyl Alcohol Copolymer Active Food Packaging Film

A method for detecting geraniol and α-terpilenol incorporated into ethylene-vinyl alcohol (EVOH) active food packaging film was established by using gas chromatography-mass spectrometry (GC-MS). The extraction and detection conditions were optimized. The external standard method was used to conduct quantitative analysis. The results showed that the GC-MS method had a good linearity over the concentration range of 0.02–2.0 mg/L for geraniol and α-terpilenol with correlation coefficients of 0.999 2 and 0.999 0, respectively. The limits of detection (LOD) for the analytes were 0.003 and 0.007 mg/kg and limits of quantification (LOQ) were 0.010 and 0.020 mg/kg, respectively. The recoveries of the preservatives were in the range of 82.5%–98.5% at spiked levels of 0.1, 0.2 and 1.0 mg/kg with relative standard deviations (RSDs) ranging from 3.6% to 10.4%. The method is rapid, simple and highly sensitive. It can provide a foundation for further insight into the release mechanism of active substances from active food packaging materials and offer a detection method for ensuring the safety of active food packaging film for application.