Prevalence and Characterization of Salmonella Isolated from Chicken Meat in Turkey |
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Authors: | Belgin Siriken Haldun Türk Tuba Yildirim Belma Durupinar Irfan Erol |
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Affiliation: | 1. Dept. of Water Products Diseases, Faculty of Veterinary Medicine, Ondokuz Mayis Univ, Samsun, Turkey;2. Ondokuz May?s ?l?e G?da ve Tar?m Hayvanc?l?k Müdürlü?ü, Hükümet Kona??, Ondokuz May?s, Samsun, Turkey;3. Dept. of Biology, Faculty of Science, Univ. of Amasya, Amasya, Turkey;4. Dept. of Medical Microbiology, Faculty of Medicine, Ondokuz May?s Univ, Kurupelit Campus, Samsun, Turkey;5. President of Food Control, Ministry of Food Agriculture and Livestock, Ankara, Turkey |
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Abstract: | This study was conducted in a Turkish province to investigate the presence of Salmonella spp. in 150 chicken meat samples using 2 phenotyping techniques: classic culture technique (CCT) and immunomagnetic separation (IMS). For the confirmation of the isolates at molecular levels, invA gene was detected in these isolates. The presence of invA, class 1 (Cls1) integrons, and integrase (Int1) genes was demonstrated by PCR assay; and the resistance of the isolated Salmonella spp. strains to antibiotics was determined by disk diffusion test. All the cultural and PCR results were evaluated together; Salmonella spp. were detected in a total of 64 (42.66%) chicken meat samples. Contamination rate was higher in carcasses (53.33%, n = 75) than in meat pieces (32%, n = 75). When results of standard culture were compared with IMS technique, IMS (n = 54) showed a clear superiority over the CCT (n = 38). A very high resistance rate (≥89.28%) to vancomycin, tetracycline, streptomycin, or nalidixic acid was found. Trimethoprim‐sulfamethoxazole resistance was present in 32.14%. Relatively lower incidence of resistance (≤8.33%) to gentamicin, chloramphenicol, ampicillin, and ceftriaxone was observed. Concurrent resistance to at least 4 antibiotics was detected in 92.85% of the isolates. Cls1 integrons and Int1 were positive in 80.95% and 95.23% of the isolates, respectively. However, Int1 alone was detected in 15.47% (n = 13). In conclusion, the high prevalence of Salmonella spp. in chicken meat may pose a potential public health risk, and the presence of antibiotic‐resistant Salmonella spp. isolate together with Cls1 integron and/or integrase might play an important role in horizontal antibiotic gene transfer. |
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Keywords: | antibiotic resistance chicken meat integron Salmonella |
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