| 出芽短梗霉产黑色素发酵工艺优化 |
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| 引用本文： | 魏永昌,袁凯凯,李振海,仝雅娜,乔长晟.出芽短梗霉产黑色素发酵工艺优化[J].食品研究与开发,2021,42(3):170-176. |
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| 作者姓名： | 魏永昌 袁凯凯 李振海 仝雅娜 乔长晟 |
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| 作者单位： | 天津科技大学生物工程学院;天津市工业微生物重点实验室;天津北洋百川生物技术有限公司;天津市设施农业研究所 |
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| 基金项目： | 天津市科技计划项目(17YFCZZC00310)。 |
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| 摘 要： | 该文以出芽短梗霉(Aureobasidium pullulans)为生产菌株,通过单因素和响应面试验对培养基组分进行优化以提高黑色素产量。结果表明,出芽短梗霉发酵产黑色素的最佳培养基组成为:蔗糖98.9 g/L、玉米浆干粉9.7 g/L、CaCl2·2H2O 2.0 g/L、NaCl 2.0 g/L、ZnSO41.0 g/L、pH 5.5。在此条件下,经过144 h发酵,黑色素产量达到17.56 g/L,相比优化之前提高了42.8%。
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| 关 键 词： | 出芽短梗霉 黑色素 发酵工艺 培养基优化 响应面 |
| 收稿时间： | 2020/9/14 0:00:00 |
Optimization of Fermentation Technology of Melanin from Aureobasidium pullulans |
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| WEI Yong-chang,YUAN Kai-kai,LI Zhen-hai,TONG Ya-n,QIAO Chang-sheng.Optimization of Fermentation Technology of Melanin from Aureobasidium pullulans[J].Food Research and Developent,2021,42(3):170-176. |
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| Authors: | WEI Yong-chang YUAN Kai-kai LI Zhen-hai TONG Ya-n QIAO Chang-sheng |
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| Affiliation: | (College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China;Tianjin Key Laboratory of Industrial Microbiology,Education Ministry Key Laboratory of Industrial Fermentation Microbiology,Tianjin 300457,China;Tianjin Peiyang Biotrans Biotechnology Company,Tianjin 300457,China;Tianjin Facility Agriculture Research Institute,Tianjin 300112,China) |
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| Abstract: | Based on a strain of Aureobasidium pullulans,an optimization on medium components was performed by means of single-factor experiment and response surface method to improve melanin yield.The results showed that the optimum medium components were as below that sucrose 98.9 g/L,corn steep flour 9.7 g/L,CaCl2·2H2O 2.0 g/L,NaCl 2.0 g/L,ZnSO41.0 g/L,pH 5.5.Under above described conditions,a desirable melanin yield of 17.56 g/L was achieved after 144 h fermentation which was 42.8%up than that before optimization. |
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| Keywords: | Aureobasidium pullulans melanin fermentation technology optimization of culture medium response surface method |
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