| 两步荧光法检测牛乳中大肠杆菌的研究 |
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| 引用本文： | 付丽娜,刘宁,霍贵成. 两步荧光法检测牛乳中大肠杆菌的研究[J]. 食品科学, 2007, 28(2): 238-240 |
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| 作者姓名： | 付丽娜 刘宁 霍贵成 |
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| 作者单位： | 东北农业大学乳品科学教育部重点实验室； 东北农业大学乳品科学教育部重点实验室 黑龙江哈尔滨150030； 黑龙江哈尔滨150030； |
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| 摘 要： | 本文主要进行了两步荧光检测牛乳中大肠杆菌的研究,确定了两步荧光法的基本条件和过程。其过程为将大肠杆菌菌悬液在TB培养基中进行12h的预培养后,取出0.1ml菌液加入到1.5ml的离心管中,再加入0.2mg/mlMUG底物0.1ml和100μg/ml的多粘菌素B0.05ml培养45min,最后加入pH10.4的0.2mol/LGly-NaOH缓冲液1.75ml终止反应,使用荧光分光光度计测荧光密度。空白样为0.1mlmol/LUG底物加0.05ml100ug/ml的多粘菌素B,再加0.2mol/LpH10.4Gly-NaOH缓冲液1.75ml。
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| 关 键 词： | 两步荧光法 检测 大肠杆菌 |
| 文章编号： | 1002-6630（2007）02-0238-03 |
| 修稿时间： | 2005-12-17 |
Study on E. coli Detection in Cowmilk by Two-Step Fluorescent Method |
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| FU Li-na,LIU Ning,HUO Gui-cheng. Study on E. coli Detection in Cowmilk by Two-Step Fluorescent Method[J]. Food Science, 2007, 28(2): 238-240 |
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| Authors: | FU Li-na LIU Ning HUO Gui-cheng |
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| Affiliation: | Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | The assay conditions and procedure were studied in this paper. Firstly, Escherichia coli was pre-incubated in TB for 12 hours. Then 0.1ml aliquots were taken to be combined with 0.05ml polymyxin B(100μg/ml) and 0.1ml MUG(4-methylum belli feryl-β-D-glucuro nide)(0.2mg/ml) and incubated at 37℃ for 45min. Finaly 1.75ml Gly-NaOH buffer(0.2mol/L, pH10.4) were added to end the reaction. The the two-step fluorescent method is better than that of MPN(most probable number), which is widely used for the detection of Escherichia coli,and was quantitatively close to the traditional MPN method. |
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| Keywords: | two-step fluorescent method detection Escherichia coli |
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