Modified PMMA monosize microbeads for glucose oxidase immobilization |
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Affiliation: | 1. School of Chemical Engineering, Shaanxi Key Laboratory of Degradable Medical Material, Northwest University, Xi''an 710069, Shaanxi, China;2. Department of Chemistry, Hong Kong Baptist University, Waterloo Road, Kowloon Tong, Hong Kong, China;1. Babes-Bolyai University, Institute of Interdisciplinary Research in Bio-Nano-Sciences, Cluj-Napoca, Romania;2. Babeş-Bolyai University, Faculty of Physics, National Centre of Magnetic Resonance, Cluj-Napoca, Romania;1. Textile Engineering Department, Amirkabir University, P.O. Box: 15874-4413, Tehran, Iran;2. Department of Organic Colorants, Institute for Color Science and Technology, P.O. Box:16765-654, Tehran, Iran;3. Center of Excellence for Color Science and Technology, Institute for Color Science and Technology, P.O. Box:16765-654, Tehran, Iran |
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Abstract: | Glucose oxidase (GOD) was immobilized onto modified polymethylmethacrylate (PMMA) microspheres by covalent bonding. Monosize PMMA microbeads with 1.5 μm diameter were produced by dispersion polymerization of methylmethacrylate by using polyvinyl alcohol as a stabilizer. Hydroxyl groups on the microbeads were first converted to aldehyde groups by periodate oxidation. Three amino compounds, namely ammonium hydroxide, ethylene diamine and hexamethylene diamine were incorporated through the aldehyde groups. Then, GOD molecules were immobilized through the spacer-arms by using glutaraldehyde. The highest amount of immobilization and activity were obtained in which hexamethylene diamine was used as the spacer-arm with 14 atom length, and were 2.1 mg g−1 polymer and 129 IU g−1 polymer, respectively. The optimal conditions for GOD immobilization were obtained as follows: pH, 6.0; temperature, 30 °C; immobilization time, 60 min; and GOD initial concentration, 0.10 mg ml−1. The optimal conditions for the GOD-immobilized PMMA microbeads were at pH 6.0 and at a temperature of 30 °C. The Km and Vmax values of the GOD-immobilized PMMA microbeads were, 13.79 mM and 26.31 mM min−1 calculated by non-linear regression, respectively. |
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