响应面法优化紫苏籽超声辅助提取原花青素工艺研究

为充分利用紫苏资源，优化其籽粕原花青素提取工艺，考察了料液比、乙醇体积分数、超声功率、温度、时间对原花青素得率的影响，并根据Box-Behnken试验设计原理，在单因素试验基础上选择主要影响因素进行响应面试验。响应面优化后提取工艺条件为乙醇体积分数70%、浸提时间0.5 h、浸提温度70 ℃、超声功率100 W、料液比1∶15。在此条件下原花青素得率理论值为0.232%，实测值为0.229%。

Optimization of Ultrasound-Assisted Extraction of Proanthocyanidins from Perilla frutescens Using Response Surface Methodology

The ultrasound-assisted extraction of proanthocyanidins from defatted seed meal of Perilla frutescens was
optimized in the present work. The effects of solid-to-liquid ratio, ethanol concentration, ultrasound power, temperature and
extraction time on the extraction yield of proanthocyanidins were evaluated. Main influencing factors were selected based on
the results of single factor experiments and optimized using response surface methodology according to the principle of Box-
Behnken design. The optimal extraction conditions were determined as follows: extraction with a 15-fold volume of 70%
aqueous ethanol solution at 70 ℃ with an ultrasound power of 100 W for 0.5 h. Under these conditions, the theoretically
predicted yield of procyanidins was 0.232%, and the actually measured value was 0.229%.