| 硒化乳酸菌胞外多糖对小鼠腹腔巨噬细胞及肿瘤细胞内游离Ca2+的影响 |
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| 引用本文： | 刘 鹭,潘道东,丁 琳,曾小群.硒化乳酸菌胞外多糖对小鼠腹腔巨噬细胞及肿瘤细胞内游离Ca2+的影响[J].食品科学,2014,35(1):250-253. |
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| 作者姓名： | 刘 鹭 潘道东 丁 琳 曾小群 |
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| 作者单位： | 宁波大学海洋学院食品系，浙江 宁波 315211 |
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| 基金项目： | 浙江省自然科学基金重点项目（Z3110211）；国家自然科学基金项目（41276121）；宁波市重大攻关项目（2011C11017） |
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| 摘 要： | 目的：研究硒化乳酸菌胞外多糖对小鼠腹腔巨噬细胞、SGC7901胃癌细胞及海拉细胞（Hela cells）内游离Ca2+的影响。方法：分离制备小鼠腹腔巨噬细胞，利用Ca2+荧光探针Fluo-3/AM及激光共聚显微镜测定加药后10 min细胞内Ca2+浓度的变化。结果：添加硒化多糖后，巨噬细胞内Ca2+浓度缓慢升高，而胃癌细胞与Hela细胞内Ca2+水平均有不同程度的下降。结论：通过对乳酸菌胞外多糖进行硒化修饰可增强其刺激巨噬细胞的能力，因而发挥其免疫功效。硒化多糖与肿瘤细胞表面受体结合后引起细胞内Ca2+内流的减少，提示硒化后的多糖可引起肿瘤细胞不同程
度的细胞凋亡。
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| 关 键 词： | 胞外多糖 硒化 巨噬细胞 钙离子 肿瘤细胞 |
| 收稿时间： | 2013-03-27 |
Effect of Selenium-Modified Exopolysaccharide from Lactococcus lactis subsp. lactis on [Ca2+]i in Mouse Macrophages and Cancer Cells |
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| LIU Lu,PAN Dao-dong,DING Lin,ZENG Xiao-qun.Effect of Selenium-Modified Exopolysaccharide from Lactococcus lactis subsp. lactis on [Ca2+]i in Mouse Macrophages and Cancer Cells[J].Food Science,2014,35(1):250-253. |
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| Authors: | LIU Lu PAN Dao-dong DING Lin ZENG Xiao-qun |
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| Affiliation: | Department of Food Science and Engineering , College of Marine, Ningbo University, Ningbo 315211, China |
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| Abstract: | Objective: The effect of Se-exopolysaccharide (EPS) from Lactococcus lactis subsp. lactis on intracellular free
Ca2+ levels in mouse macrophages cells, gastric cancer cells SGC7901 and Hela cells was studied. Methods: Cells labeled
with Fluo-3/AM dye for 10 min were examined using laser confocal scanning microscope. Results: The intracellular Ca2+
level in mouse macrophages increased in the presence of Se-EPS. On the other hand, Se-EPS could decrease the intracellular
Ca2+ levels both in gastric cancer cells SGC7901 and Hela cells. Conclusion: Se-EPS can activate macrophages, which may
be related to Ca2+ signal pathways. Se-rich EPS has gained the capacity to induceapoptosis in tumor cells. |
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| Keywords: | exopolysaccharides selenization macrophage Ca2+ cancer cells |
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