酶法预处理压榨月见草油工艺的研究

以月见草籽为原料，采用酶法预处理后再压榨的方法制取月见草油，先用复合酶（复合多糖酶、植物水 解酶、纤维素酶比为4∶1∶1）对月见草籽进行前处理，通过单因素和正交试验确定最佳酶解条件为酶解温度50 ℃、 酶解pH 5.0、酶用量2%、酶解时间5.5 h，在此条件下游离油得率24%，然后将含油物料烘干后调整水分至9%，以 60 ℃为初始入榨温度进行压榨，最后得出在压榨温度90 ℃、压榨压力3.5 MPa时，出油率可达69%。制取得到的月 见草油中的γ-亚麻酸以及亚油酸等不饱和酸被很好的保留，油脂品质良好。

Optimization of Enzymatic Pretreatment Process for Pressing of Evening Primrose Oil

In this work, evening primrose oil was pressed from enzymatically pretreated samples of evening primrose
seeds. The enzymatic pretreatment was carried out using a mixture composed of a commercial complex enzyme preparation
hydrolyzing carbohydrates, viscozyme and cellulase (all from Novozymes, 4:1:1). By using one-factor-at-a-time and
orthogonal array design methods, the optimal enzymatic pretreatment conditions were determined to be hydrolysis for 5.5 h at 50 ℃,
initial pH 5.0 with a total enzyme dosage of 2%. Under these conditions, the yield of free oil was 24%. The pretreated material
was dried until the moisture content reached 9%, and then pressed initially at 60 ℃, finally at 90 ℃ and 3.5 MPa, resulting in an oil
yield of 69%. γ-Linolenic, linoleic and some other unsaturated fatty acids were retained at significant levels in the evening
primrose oil, and the oil quality was good.