顶空固相微萃取与气质联用法分析酵母浸膏辐照挥发性成分

采用顶空固相微萃取-气相色谱-质谱联用法分析酵母浸膏辐照后的挥发成分。结果表明：未经辐照的酵母浸膏萃取得到13 种挥发性成分，其中含酸类1 种、醛类2 种、醇类2 种、烯烃类1 种、含氮化合物3 种，主要成分为乙酸、吲哚、2-乙基己醇。辐照后的酵母浸膏萃取得到35 种挥发性成分，其中酸类1 种、醛类7 种、醇类2 种、烯烃类4 种、酮类2 种、含氮化合物14 种、含硫化合物2 种、酯类1 种、苯类2 种。挥发物的种类和含量均随辐照剂量的增加而增加。

Analysis of Volatile Compounds from Irradiated Yeast Extract by Headspace Solid Phase Micro-Extraction Coupled with Gas Chromatography-Mass Spectrometry

A method for determining volatile compounds from irradiated yeast extract using headspace solid phase
microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) was developed in this paper. The
results showed that 13 volatile compounds were detected from unirradiated yeast extract including 1 acid, 2 aldehydes, 2
alcohols, 1 alkene and 3 nitrogen compounds and the major compounds were acetic acid, indole and 2-ethyl-1-hexanol.
Totally 35 volatile compounds including 1 acid, 7 aldehydes, 2 alcohols, 4 alkenes, 2 ketones, 14 nitrogen compounds, 2
sulfur compounds, 1 ester and 2 benzenes were detected from irradiated yeast extract. The number and contents of volatile
compounds increased with increasing irradiation dosage.