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| 葡萄酒中赭曲霉素A测定方法的研究 | |
| 引用本文： | 康健,钟其顶,熊正河,肖冬光,张辉.葡萄酒中赭曲霉素A测定方法的研究[J].食品与发酵工业,2009,35(3). |
| 作者姓名： | 康健 钟其顶 熊正河 肖冬光 张辉 |
| 作者单位： | 1. 天津科技大学,天津,300457;中国食品发酵工业研究院,北京,100027 2. 中国食品发酵工业研究院,北京,100027 3. 天津科技大学,天津,300457 4. 中粮酒业有限公司,北京,100005 |
| 摘 要： | 采用固相萃取小柱对葡萄酒中的赭曲霉素A(OTA)进行浓缩处理,选择高效液相荧光法和超高效液相质谱联用法,分别测定葡萄酒中的OTA含量。采用HPLC-FLD法测定葡萄酒中OTA时,线性良好,R2=0.9997;经固相萃取小柱浓缩后,测定方法检出限可达到0.002μg/L;回收率为85.7%～102.6%;RSD(n=5)为4.5%。通过HPLC-FLD和UPLC-MS/MS测定OTA方法对比发现HPLC-FLD方法具有更好的灵敏度和检出限。 |
| 关 键 词： | 赭曲霉素A 葡萄酒 高效液相-荧光检测器 超高效液相色谱/质谱联用 |
Comparison of Methods for the Determination of Ochratoxin A in Wines |
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| Kang Jian,Zhong Qiding,Xiong Zhenghe,Xiao Dongguang,Zhang Hui.Comparison of Methods for the Determination of Ochratoxin A in Wines[J].Food and Fermentation Industries,2009,35(3). | |
| Authors: | Kang Jian Zhong Qiding Xiong Zhenghe Xiao Dongguang Zhang Hui |
| Abstract: | In accordance with the nature of wine and OTA,we performed sample clean-up utilizing solid-phase extraction(SPE).The detection of wine OTA was accomplished using high-performance liquid chromatography(HPLC) combined with fluorescence detection(FLD),and ultra performance liquid chromatography(UPLC) combined with Tandem Quadrupole(MS/MS).Detection was validated by the clear linearity(R2=0.999 7) and the detection limit of OTA in wine were 0.004 g/L after concentration;recovery rate was 85.7%～102.6 %,and precision(RSD=4.5%,n=5).The comparison of HPLC-FLD and UPLC-MS/MS in terms of sensitivity and detection limit led to the conclusion that HPLC-FLD offered better results in the sub-ppb concentration level and higher sensitivity. |
| Keywords: | ochratoxin A wine HPLC-FLD UPLC-MS/MS |
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