Application of silica gel-sintered plate to thin layer lipid chromatographic analysis

The merits and disadvantages of using the silica gel-sintered plate for lipid chromatographic analysis were investigated in detail. The commercially available sintered plate could be used repeatedly, employing the reconditioning procedure which involved chromic-sulfuric acid treatment and subsequent activation. The reconditioned sintered plate has now been used successfully 20 times, for lipid analysis without any deterioration of the excellent resolution power for complex lipid mixtures for all the solvent systems. Since the sintered plate is sturdy, the chromatogplate could be immersed directly in the liquid reaction mixture, so that spots on the chromatogram could be seen and impregnated plates prepared simply. The sintered plate was found to be much more economical than the silica gel-coated plate. Presented in part at the Japan Oil Chemists' Society 20th Annual Meeting, Tokyo, November 1972 The following abbreviations are used in this paper: TLC, thin layer chromatography; PA, phosphatidic acid; PS, phosphatidylserine; PC, phosphatidylcholine; LPC, lysophosphatidylcholine; PI, phosphatidylinositol; SPM, sphingomyelin; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; CL, cardiolipin; CMH, ceramide-monohexoside; CDH, ceramide-dihexoside; CTH, ceramide-trihexoside; NL, neutral lipid.