Preparation of diacylglycerol‐enriched palm olein by phospholipase A1‐catalyzed partial hydrolysis |
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Authors: | Yong Wang Mouming Zhao Shiyi Ou Keke Song Xue Han |
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Affiliation: | 1. College of Light Industry and Food Science, South China University of Technology, Guangzhou, China;2. Department of Food Science and Engineering, Jinan University, Guangzhou, China |
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Abstract: | Partial hydrolysis of palm olein catalyzed by phospholipase A1 (Lecitase Ultra) in a solvent‐free system was carried out to produce diacylglycerol (DAG)‐enriched palm olein (DEPO). Four reaction parameters, namely, reaction time (2–10 h), water content (20–60 wt‐% of the oil mass), enzyme load (10–50 U/g of the oil mass), and reaction temperature (30–60 °C), were investigated. The optimal conditions for partial hydrolysis of palm olein catalyzed by Lecitase Ultra were obtained by an orthogonal experiment as follows: 45 °C reaction temperature, 44 wt‐% water content, 8 h reaction time, and an enzyme load of 34 U/g. The upper oil layer of the reaction mixture with an acid value of 54.26 ± 0.86 mg KOH/g was first molecularly distilled at 150 °C to yield a DEPO with 35.51 wt‐% of DAG. The DEPO was distilled again at 250 °C to obtain a DAG oil with 74.52 wt‐% of DAG. The composition of the acylglycerols of palm olein and the DEPO were analyzed and identified by high‐performance liquid chromatography (HPLC) and HPLC/electrospray ionization/mass spectrometry. The released fatty acids from the partial hydrolysis of palm olein catalyzed by phospholipase A1 showed a higher saturated fatty acid content than that of the raw material. |
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Keywords: | Diacylglycerol Palm olein Phospholipase A1 Partial hydrolysis |
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