Potent Triple Helix Stabilization by 5′,3′‐Modified Triplex‐Forming Oligonucleotides |
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Authors: | Nouha Ben Gaied Dr Zhengyun Zhao Dr Simon R Gerrard Keith R Fox Prof Tom Brown Prof |
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Affiliation: | 1. School of Chemistry, University of Southampton, Highfield, SO17 1BJ, Southampton (UK), Fax: (+44)?2380592991;2. School of Biological Sciences, University of Southampton, Bassett Crescent East, SO16 7PX, Southampton (UK) |
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Abstract: | Anthraquinone and pyrene analogues attached to the 3′ and/or 5′ termini of triplex‐forming oligonucleotides (TFOs) by various linkers increased the stability of parallel triple helices. The modifications are simple to synthesize and can be introduced during standard solid‐phase oligonucleotide synthesis. Potent triplex stability was achieved by using doubly modified TFOs, which in the most favourable cases gave an increase in melting temperature of 30 °C over the unmodified counterparts and maintained their selectivity for the correct target duplex. Such TFOs can produce triplexes with melting temperatures of 40 °C at pH 7 even though they do not contain any triplexstabilizing base analogues. These studies have implications for the design of triplex‐forming oligonucleotides for use in biology and nanotechnology. |
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Keywords: | anthraquinone DNA recognition DNA pyrene triple helix |
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