Chivosazoles A and F,Cytostatic Macrolides from Myxobacteria,Interfere with Actin |
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| Authors: | Randi Diestel Herbert Irschik Dr Rolf Jansen Dr Mohammed W Khalil Dr Hans Reichenbach Prof Dr Florenz Sasse Dr |
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| Affiliation: | 1. Abteilung Chemische Biologie, Helmholtz‐Zentrum für Infektionsforschung, 38124 Braunschweig (Germany), Fax: (+49)?531‐6181‐3499;2. Arbeitsgruppe Mikrobielle Wirkstoffe, Helmholtz‐Zentrum für Infektionsforschung, 38124 Braunschweig (Germany);3. formerly Abteilung Naturstoffbiologie, GBF now Helmholtz‐Zentrum für Infektionsforschung, 38124 Braunschweig (Germany);4. Present address: Fayoum University, Fayoum (Egypt) |
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| Abstract: | Chivosazoles A and F, isolated from Sorangium cellulosum, showed high antiproliferative activity with different mammalian cell lines including human cancer cells. The chivosazoles caused a delay in G2/M phase of the cell cycle, and treated cells often contained two nuclei. By labeling F‐actin it was shown that the actin cytoskeleton of the cells starts to break down after a few minutes of treatment. In vitro polymerization assays with purified G‐actin revealed that the chivosazoles inhibit actin polymerization and also cause a depolymerization of pyrene‐labeled F‐actin microfilaments prepared in vitro. Chivosazoles are new tools for the investigation of issues concerning the actin cytoskeleton and they have a different mode of action from the known microfilament‐disrupting compounds like rhizopodin and cytochalasin D. |
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| Keywords: | actin biological activity cell cycle chivosazole cytochalasin rhizopodin |
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