地黄色素的提取及稳定性研究

目的:对地黄色素的提取方法和稳定性进行初步研究。方法:以地黄的新鲜块根为原料,利用超声波技术,用不同的有机溶剂提取鲜地黄黄色素,采用正交试验的方法选取最佳工艺;考察在不同pH值、温度、紫外线、氧化剂、还原剂、金属离子等条件下鲜地黄的色素。结果:当物料与无水乙醇的比例为1:3,温度为40℃,超声10min,收率为6．6%。鲜地黄色素在碱性条件下稳定,紫外线和还原剂对其稍有影响,热、氧化剂及几种金属离子对色素有较大影响。结论:最佳提取工艺条件确立。色素有一定稳定性。

Study on Pigment Extraction from Rehmannia glutinosa with Ultrasound and Its Stability

Objective:The fresh glutinous Rehmannia pigment extract and its stability were studied.Method:Fresh Rehmannia glutinosa’s fresh block root was taken as raw material and extracted by organic solvent using ultrasound.The optimum extracting conditions were got with orthogonal test.Result:The optimum conditions are temperature 40℃,treating for 10 min with ultrasound and ratio of material to ethanol absolute 1:3(W/V).Under these conditions,the extraction rate is 6.6%.Results showed that the pigment is stable on alkalesccnce condition,and pH value,heating,oxidants and some metal irons have tremendous effects on it, but ultraviolet radiation and antioxidants have little effects on it.Conclusion:The optimum extraction conditions Rehmannia glutinosa pigment are established by single factor test and orthogonal test.The stability of the pigment is confirmed.