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枇杷多糖的提取纯化及理化性质初步研究
引用本文:庞振凌,田龙. 枇杷多糖的提取纯化及理化性质初步研究[J]. 食品研究与开发, 2007, 28(4): 14-16
作者姓名:庞振凌  田龙
作者单位:南阳师范学院生命科学与技术学院,河南,南阳,473061
摘    要:建立了枇杷多糖的提取纯化方法,并对提取的枇杷多糖进行了理化性质分析。枇杷多糖经40%乙醇分级得到的组分kns5,用Sephadex G-150凝胶柱层析得到单一峰,Lens5的酸性水解液经纸层析和薄层层析分析,其单糖组成为葡萄糖和木糖,用Sephadex G-150凝胶柱层析法测得其分子量为43000Da。

关 键 词:枇杷  多糖  分离纯化  理化性质
修稿时间:2006-07-26

STUDY ON ISOLATION PURIFICATION AND CHARACTERISTICS STUDY ON LOQUAT POLYSACCHARIDE
PANG Zhen-ling,TIAN Long. STUDY ON ISOLATION PURIFICATION AND CHARACTERISTICS STUDY ON LOQUAT POLYSACCHARIDE[J]. Food Research and Developent, 2007, 28(4): 14-16
Authors:PANG Zhen-ling  TIAN Long
Affiliation:Institute of Life Science and Technology, Nanyang Normal College,Nanyang 473061, Henan,China
Abstract:In this study a method of Loquat polysaccharide isolation and purification was setup and the physical and chemical characteristics of Loquat polysaccharide was also analyzed.Product Len5 was obtained when Loquat polysaccharide was classified with 40 % ethanol, and a single apex was obtained in the Sephadex G-150 gel chromatography analysis. The monosaccharide components of the acidic solution of Lens5 were identified as glucose and xylose according to the results of paper chromatography and thin layer chromatography analysis.The molecular weight of Len5 is 43 000 Dalton according to the result of Sephadex G-150 gel chromatography analysis.
Keywords:Loquat   polysaccharide   isolation and purification   physical and chemical characteristic
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