Enhanced degradation of lignin in corn stalk by combined method of Aspergillus oryzae solid state fermentation and H2O2 treatment |
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| Affiliation: | 1. Institute of Agro-Production Processing Engineer, Jiangsu University, Zhenjiang 212013, PR China;2. Beijing Green Technology and Natural Biotechnology Co. Ltd., Beijing 102300, PR China;1. College of Environmental Science and Engineering, Hunan University, Changsha, Hunan 410082, China;2. Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, Hunan 410082, China;1. Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand;2. School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur 613401, India;3. Department of Biotechnology, College of Science and Humanities, Ramapuram Campus, SRM Institute of Science and Technology, Bharathi Salai, Ramapuram, Chennai 600089, India;4. Innovative Green Product Synthesis and Renewable Environment Development Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam;5. Academy of Science, Royal Society of Thailand, Bangkok 10300, Thailand;1. Department of Food Science and Technology, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China;2. Department of Biology, Lakehead University, Ontario P7B 5E1, Canada;3. Co-innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China;4. Institute of Agricultural Products Processing, Shanxi Academy of Agricultural Science, Tianyuan 030031, China;1. National Engineering and Technology Research Center of Wood-based Resources Comprehensive Utilization, Zhejiang A & F University, Hangzhou, Zhejiang, 311300, China;2. Department of Chemistry, Zhejiang A & F University, Hangzhou, Zhejiang, 311300, China;3. Shanghai Synchrotron Radiation Facility (SSRF), Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, China;4. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China |
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| Abstract: | To shorten the time of corn stalk pretreatment and increase the degradation of lignin, solid state fermentation (SSF) with Aspegillus oryzae CGMCC5992 in the presence of H2O2 was carried out to degrade lignin in stalk. In this study, the conditions for lignin hydrolysis catalyzed by enzymes produced in SSF for 10 days were optimized by mono-factor-at-a-time design and response surface method (RSM). The removal rate of lignin increased from 30% (before hydrolysis) to 80.3% (after hydrolysis) under the temperature of 55 °C, pH 6.0, water/stalk ratio of 40, and the concentration of H2O2 at 4% (w/w). In comparison, the removal rate of lignin after 50 days of SSF only reached 57.8%. Proteomic analysis provided support for the increased lignin hydrolysis. Fermentation with A. oryzae CGMCC5992 in the presence of H2O2 increased the amount of peroxidase and intracellular catalase and decreased the amount of extracellular catalase. Therefore, the method introduced in this study can significantly shorten the time of SSF and increase the removal rate of lignin. |
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| Keywords: | Corn stalk Lignin degradation Enzyme Proteomic analysis |
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