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Bio-methane potential test (BMP) using inert gas sampling bags with macroalgae feedstock
Affiliation:1. Institute for Agricultural Engineering and Animal Husbandry, Bavarian State Research Center for Agriculture, Am Staudengarten 3, 85354 Freising, Germany;2. Department of Agriculture and Food Economy, Hochschule Weihenstephan-Triesdorf, Am Staudengarten 1, 85354 Freising, Germany;3. Department of Experimental Farm, Bavarian State Research Center for Agriculture, Lange Point 12, 85354 Freising, Germany;4. Chair of Urban Water Systems Engineering, Technical University of Munich, Am Coulombwall 3, 85748 Garching, Germany;1. Austrian Centre of Industrial Biotechnology, Petersgasse 14/5, A-8010 Graz, Austria;2. Engineering Consultant, Wiedner Hauptstrasse 90/2/19, A-1050 Vienna, Austria;3. IPUS Mineral- und Umwelttechnologie GmbH, Werksgasse 281, A-8786 Rottenmann, Austria;4. Medical University of Graz, Centre for Medical Research, Core Facility Molecular Biology, Stiftingtalstraße 24, A-8010 Graz, Austria;5. Medical University of Graz, Centre for Medical Research, Core Facility Computational Bioanalytics, Bioinformatics, Stiftingtalstraße 24, A-8010 Graz, Austria;6. University of Natural Resources and Life Sciences, Institute of Environmental Biotechnology, Konrad Lorenz Strasse 20, A-3430 Tulln, Austria;1. Centre for Rapid and Sustainable Product Development, Polytechnic of Leiria, Rua de Portugal – Zona Industrial, Marinha Grande 2430-028, Portugal;2. Universidad Estatal Amazónica, km. 2. 1/2 vía Puyo a Tena (Paso Lateral), Puyo, Pastaza 160150, Ecuador;3. U.S. Environmental Protection Agency, Office of Research and Development, 26 W. Martin L. King Dr. Cincinnati, OH 45268, USA;4. Chemical Engineering Graduate Program, University of Atlántico, Puerto Colombia 080007, Colombia;1. AMS Envolure, 1682 rue de la Valsière, 34184 Montpellier Cedex 4, France;2. SIAAP, Direction du Développement et de la Prospective, 82 Avenue Kléber, 92700 Colombes, France;3. SCANAE, 1682 rue de la Valsière, 34790 Grabels, France;4. Centre de Recherche Royallieu, Université Technologique de Compiègne, BP 20529, rue Personne de Roberval, 60205 Compiègne Cedex, France
Abstract:An approach to Bio-methane potential test (BMP) was carried out at mesophilic temperature of 35 °C with Supel? inert gas sampling bags as biogas collection and storage bags, using selected seaweed (macroalgae) as substrate. Samples were given a range of pre-treatments from washing, drying and macerating. Dried laminaria digitata (DD) with 68.14% VS (%TS) produced the highest BMP of 141 ± 5.77 L CH4/kg VS, with methane content increasing to about 70%, while the lowest BMP of 93.35 ± 5.03 L CH4/kg VS with methane content of about 65% was obtained for fresh laminaria digitata (FD) with 72.03% VS (%TS). Methane yields of 97.66 and 67.24 m3 CH4/t wet weight based on BMP results were obtained for DD and FD. Both DD and FD achieved within 28% and 38% of the theoretical BMP value based on the Buswell equation, respectively. The total methane (V) produced was computed based on;V = X1 + X2 – X3 corrected to Standard temperature and pressure (STP).where X1 = daily calculated headspace methane volume, X2 = daily measured volume of methane in gas bags, X3 = previous day headspace methane volume. An advantage of this approach is the volumetric measurement of gas produced directly from the gas bags, hence it does not require liquid displacement or pressure transducers. Results from a second set of freshly collected sample seaweed sample showed it was in agreement with published BMP values. All analysis were carried out without mineral supplementation.
Keywords:Biogas  Biodegradability  BMP  Seaweed  Methane  Algae
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