Lipase immobilized carbon nanotubes for conversion of Jatropha oil to fatty acid methyl esters |
| |
| Affiliation: | 1. Nanotechnology Lab, Division- H1, Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30, Chandigarh 160030, India;2. Optical Devices and Systems Division, Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30, Chandigarh 160030, India;1. Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia;2. Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia;3. Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo 12311, Egypt;1. Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran;2. Research and Technology Institute of Plant Production (RTIPP), Shahid Bahonar University of Kerman, Kerman, Iran;3. Department of Nanotechnology, Graduate University of Advance Technology, Kerman, Iran |
| |
| Abstract: | Lipase has been immobilized on multi-walled carbon nanotubes (MWCNTs) through the reaction of the carboxylated nanotubes with the enzyme in the presence of N-hydroxysulfosuccinimide (NHS) and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride. Successful immobilization has been verified by infrared spectroscopy and scanning electron microscopy. The synthesized biomaterial has been used for catalyzing the conversion of Jatropha oil to the fatty acid methyl esters (FAME). Progress of the conversion reaction has been monitored with NIR spectroscopy. Gas chromatographic studies have indicated that the use of lipase–MWCNT bioconjuagate leads to almost quantitative conversion of Jatropha oil to the FAME. Around 1 h of the reaction, in the presence of around 15% of catalyst (mass fraction of catalyst on the total mixture), has yielded the desired results. The above used catalyst support has been regenerated 10 times without any adverse effect on the enzyme activity. |
| |
| Keywords: | Bioimmobilized CNTs Biocatalysis Fatty acid methyl esters Jatropha curcus Enzymatic activity |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
