The growth of oleaginous Rhodotorula glutinis in an internal-loop airlift bioreactor by using mixture substrates of rice straw hydrolysate and crude glycerol |
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| Affiliation: | 1. Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia;2. University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan;3. Manufacturing and Industrial Processes Division, Faculty of Engineering, Centre for Food and Bioproduct Processing, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia;4. Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;5. Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan;6. Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan;7. Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan |
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| Abstract: | The conversion of lignocellulosic biomass (LCB) to microbial oils is attracting a growing amount of attention. However, the growth of the oleaginous yeast Rhodotorula glutinis on LCB hydrolysate (mainly rice straw) only will lead to a low lipid mass fraction, in the range of 10–20%. This study shows that the addition of crude glycerol to the LCB hydrolysate medium can efficiently raise the lipid mass fraction to the range of 30–40%. Crude glycerol is a by-product in the biodiesel production process. The use of renewable LCB hydrolysate and crude glycerol would greatly reduce the substrate cost for microbial oil production using R. glutinis. In addition, the results of experiments show that a low-cost airlift bioreactor is a more suitable fermentation process for the growth of R. glutinis than the use of a conventional agitation tank. When using mixed carbon sources of LCB hydrolysate with 30 kg m−3 of reducing sugars and 30 kg m−3 of crude glycerol, a maximal cell mass of 21.4 kg m−3 and lipid mass fraction of 58.5 ± 6.2 were achieved in an internal loop airlift bioreactor, and this process may have the potential to be applied in scale-up production. |
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| Keywords: | Hydrolysate Oleaginous Mixed carbon source Crude glycerol |
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