| 寡胺-葡聚糖类阳离子化合物合成及其在基因转染中的应用 |
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| 引用本文： | 陈建海,平渊,马守栋,江永南. 寡胺-葡聚糖类阳离子化合物合成及其在基因转染中的应用[J]. 功能材料, 2009, 40(5) |
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| 作者姓名： | 陈建海 平渊 马守栋 江永南 |
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| 作者单位： | 南方医科大学,南方医院药学部,广东,广州,510515 |
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| 基金项目： | 国家自然科学基金资助项目(30772660) |
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| 摘 要： | 应用氧化葡聚糖与精胺通过还原胺法反应,制备了聚阳离子非病毒基因载体葡聚糖-寡胺化合物;用红外光谱测定仪、核磁共振波谱仪、紫外光谱仪,激光纳米粒度测定仪、透射电子显微镜进行了表征.证实了该载体为葡聚糖-精胺化合物DSP.其平均粒径为170nm,Zeta电位为+31.1mV,镜下呈园球形状.将载体DSP与pCMV-GFP的质粒DNA以8:1(N/P)组成复合物时,对SMMC-7721人肝癌细胞进行体外转染,转染效率高于阳性对照组transfectine 2000,为31.1%.实验表明该材料有望成为临床的非病毒基因载体.
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| 关 键 词： | 葡聚糖 精胺 聚阳离子 复合物 转染率 |
Synthesis,characterization and application of oligoamide-dextran polycations as a vector for gene transfection |
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| CHEN Jian-hai , PING Yuan , MA Shou-dong , JIANG Yong-nan. Synthesis,characterization and application of oligoamide-dextran polycations as a vector for gene transfection[J]. Journal of Functional Materials, 2009, 40(5) |
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| Authors: | CHEN Jian-hai PING Yuan MA Shou-dong JIANG Yong-nan |
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| Affiliation: | Department of Pharmacy;Nanfang Hospital;Southern Medical University;Guangzhou 510515;China |
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| Abstract: | The dextran-spermine polycations(DSP) as a non-virus gene vector have been prepared by the reduced-amine reaction between oxided dextran and spermine.This structure of compounds has been proved by IR,1H-NMR spectroscopy.The particles size and zeta potential were 170nm and +31.1mV respectively in the average,measured by malvern zetasizer 3000HS instrument.The polyplexes were formed by the p CMV-GFP plasmid DNA combined with the vector when the N/P ratio of the carrier to DNA is 8 to 1.The highest yield of tr... |
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| Keywords: | dextran spermine polycation polyplex transfection efficiency |
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