| 促进丹参悬浮培养细胞生长和水溶性酚酸含量的因素 |
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| 引用本文： | 杨芳,孙磊,王继花,步营,杜旭东,刘同祥,张宗申. 促进丹参悬浮培养细胞生长和水溶性酚酸含量的因素[J]. 昆明理工大学学报(自然科学版), 2008, 33(3): 96-99 |
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| 作者姓名： | 杨芳 孙磊 王继花 步营 杜旭东 刘同祥 张宗申 |
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| 作者单位： | 大连工业大学,生物与食品工程学院,辽宁大连,116034;中央民族大学,中国少数民族传统医学研究中心,北京,100081 |
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| 基金项目： | 辽宁省教育厅资助项目 , 辽宁省大连市青年科技人才基金 |
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| 摘 要： | 研究了影响丹参悬浮细胞生长及水溶性酚酸生物合成的因素,包括激素、碳源及基本培养基等.结果表明:MS 2,4-D 0.5 mg/L有利于丹参悬浮细胞的生长,6,7-V 2,4-D0.5 mg/L有利于丹参悬浮细胞水溶性酚酸的合成;丹参细胞能有效地利用蔗糖并得到较高的丹参酚酸产量;丹参悬浮细胞的生长周期约为18 d,在16 d时收获细胞可得到最高的生物量和丹参酚酸含量.
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| 关 键 词： | 丹参 悬浮细胞 碳源 植物激素 细胞培养 |
Factors to Improve Growth and Depsides Content in Suspension Cell Culture of Salvia Miltiorrhiza |
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| YANG Fang,SUN Lei,WANG Ji-hua,BU Ying,DU Xu-dong,LIU Tong-xiang,ZHANG Zong-shen. Factors to Improve Growth and Depsides Content in Suspension Cell Culture of Salvia Miltiorrhiza[J]. Journal of Kunming University of Science and Technology(Natural Science Edition), 2008, 33(3): 96-99 |
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| Authors: | YANG Fang SUN Lei WANG Ji-hua BU Ying DU Xu-dong LIU Tong-xiang ZHANG Zong-shen |
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| Abstract: | The effects of several factors(hormones,carbon sources and different culture media) on growth and production of depsides in suspension cell culture of salvia miltiorrhiza are investigated in this paper.It is indicated through the results that the optimized medium for suspension cells growth is MS 2,4-D 0.5 mg/L,and for accumulation of depsides is 6,7-V 2,4-D 0.5 mg/L.For both suspension cells growth and accumulation of depsides,sucrose is proved to be the most suitable carbon source.The cell growth period is approximately 18-days long,and it arrives at the peak point of both biomass and depsides content by the time of 16 days. |
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| Keywords: | salvia miltiorrhiza suspension cells carbon sources phytohormones cell culture |
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