| 两种用于微型生化分析的光探测方法 |
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| 引用本文： | 李锋,吴一辉,赵华兵,鞠挥.两种用于微型生化分析的光探测方法[J].传感器与微系统,2004,23(2):18-20. |
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| 作者姓名： | 李锋 吴一辉 赵华兵 鞠挥 |
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| 作者单位： | 长春光学精密机械与物理研究所,应用光学国家重点实验室,吉林,长春,130022 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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中国科学院知识创新工程项目 |
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| 摘 要： | 针对微流控分析芯片样品剂量少与微流体芯片集成等要求,设计了两种易于集成在芯片内的,用分光光度法对混合后液体的吸收光谱进行探测的方法。实验利用460～800nm的可见光光源,采用了直接探测法和消逝波探测法对样品进行探测。通过两种方法的比较证明:直接探测法具有原理简单、消耗样品量少、结构容易实现等优点;消逝波探测法具有需要样品量极少、灵敏度高、易于集成等优点。
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| 关 键 词： | 微流控分析芯片 消逝波 自聚焦透镜 微型光纤光谱仪 |
| 文章编号： | 1000-9787(2004)02-0018-03 |
| 修稿时间： | 2003年11月20日 |
Two optical detection methods for micro biochemical analyses |
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| LI Feng,WU Yi-hui,ZHAO Hua-bing,JU Hui n Institute of Optics Fine Mechanics and Physics,Changchun ,China.Two optical detection methods for micro biochemical analyses[J].Transducer and Microsystem Technology,2004,23(2):18-20. |
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| Authors: | LI Feng WU Yi-hui ZHAO Hua-bing JU Hui n Institute of Optics Fine Mechanics and Physics Changchun China |
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| Affiliation: | LI Feng,WU Yi-hui,ZHAO Hua-bing,JU Hui n Institute of Optics Fine Mechanics and Physics,Changchun 130022,China) |
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| Abstract: | For the need of fluidic chip which consumes fewer specimens and is easy to integrate with micro-fluid chip,two kinds of spectrophotometric analysis methods are designed.Both direct detection method and evanescent wave detection method are used in the experiments by visible light(460～800 nm).The experimental results proved that direct detection method is simple and easy to realize,and to consume fewer reagents;on the other hand,evanescent wave detection method consumes far fewer reagents,and it is sensitive to the reagent and easy to integrate on the chip. |
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| Keywords: | micro fluidic chip evanescent wave self-focus lens fiber micro spectrometer |
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