| 荔枝皮原花青素低聚体的定性分析 |
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| 引用本文： | 周玮婧,隋勇,孙智达,谢笔钧.荔枝皮原花青素低聚体的定性分析[J].食品科学,2012,33(17):40-43. |
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| 作者姓名： | 周玮婧 隋勇 孙智达 谢笔钧 |
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| 作者单位： | 1.武汉市食品药品检验所
2.华中农业大学食品科学技术学院 |
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| 基金项目： | 国家自然科学基金-广东省联合基金项目(U0731005);“十一五”国家科技支撑计划项目(2006BAD27B08) |
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| 摘 要： | 制备并定性分析荔枝皮原花青素低聚体(LPOPC)。荔枝皮原花青素(LPPC)通过乙醇浸提，AB-8大孔树脂纯化，Toyopearl HW-40S 树脂层析分级处理得到LPOPC，并按聚合度分成了3个组分。通过红外光谱、高效液相色谱(HPLC)和液相色谱-质谱联用(LC-MS)等分析表明，LPOPC中主要成分为(－)-表儿茶素，并且含有A型原花青素二聚体和A型原花青素三聚体。通过选择性的级分收集，可以得到纯度较高的原花青素单体、二聚体和三聚体。
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| 关 键 词： | 荔枝皮 原花青素 低聚体 LC-MS |
| 收稿时间： | 2011-07-18 |
Qualitative Analysis of Oligomeric Procyanidins from Litchi chinensis Pericarp |
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| ZHOU Wei-jing,SUI Yong,SUN Zhi-da,XIE Bi-jun.Qualitative Analysis of Oligomeric Procyanidins from Litchi chinensis Pericarp[J].Food Science,2012,33(17):40-43. |
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| Authors: | ZHOU Wei-jing SUI Yong SUN Zhi-da XIE Bi-jun |
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| Affiliation: | 1.Wuhan Institute for Food and Drug Control,Wuhan 430012,China;2.College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China) |
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| Abstract: | Litchi (Litchi chinensis Sonn.) is a subtropical fruit of high commercial value; however, its pericarp results in a reduced market value. In the present study, oligomeric procyanidins from Litchi chinensis pericarp (LPOPC) were prepared and qualitatively analyzed. LPOPC were extracted with ethanol aqueous solution, purified by AB-8 macroporous resin chromatography, and fractionated by Toyopearl HW-40S resin chromatography to obtain LPOPC. LPOPC was found to comprise three fractions with different degrees of polymerization (DP). The results of FT-IR, HPLC and LC-MS analyses revealed that the major components of LPOPC were (－)-epicatechin, procyanidin A2, procyanidin A3. Highly pure monomer, dimmers and trimers were obtained through selective collection of fractions. |
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| Keywords: | Litchi chinensis pericarp procyanidins oligomer LC-MS |
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