Characterisation of anthocyanins in the black soybean (Glycine max L.) by HPLC-DAD-ESI/MS analysis |
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Authors: | Jin Hwan Lee Nam Suk KangSang-Ouk Shin Seong-Hyu ShinSea-Gyu Lim Duck-Yong SuhIn-Youl Baek Keum-Yong ParkTae Joung Ha |
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Affiliation: | Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA, 1085 Neidong, Milyang 627-803, Republic of Korea |
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Abstract: | The aim of this study was to isolate and identify the anthocyanins in the black seed coated soybean (cv. Cheongja 3, Glycine max (L.) Merr.) using reverse phase C-18 open column chromatography and high-performance liquid chromatography (HPLC) with diode array detection and electro spray ionization/mass spectrometry (DAD-ESI/MS) analysis, respectively. Anthocyanins were extracted from the coat of black soybeans with 1% TFA in methanol, isolated by RP-C-18 column chromatography, and their structures elucidated by 1D and 2D NMR spectroscopy. The isolated anthocyanins were characterised as delphinidin-3-O-glucoside (3), cyanidin-3-O-glucoside (5), petunidin-3-O-glucoside (6), pelargonidin-3-O-glucoside (7) and cyanidin (9). Furthermore, four minor anthocyanins were detected and identified as catechin-cyanidin-3-O-glucoside (1), delphinidin-3-O-galactoside (2), cyanidin-3-O-galactoside (4), and peonidin-3-O-glucoside (8) based on the fragmentation patterns of HPLC-DAD-ESI/MS analysis. |
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Keywords: | Anthocyanins Glycine max NMR HPLC-ESI/MS Black soybean |
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