Quantification of coumarin derivatives in Noni (Morinda citrifolia) and their contribution of quenching effect on reactive oxygen species |
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Authors: | Rie Ikeda Mitsuhiro Wada Toshiaki Nishigaki Kenichiro Nakashima |
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Affiliation: | 1. Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan;2. Tokyo Noni Research Center, 4-7-11 Ishishiba, Matsumoto 399-0007, Japan |
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Abstract: | The quantification of coumarin derivatives such as scopoletin, 7-hydroxycoumarin (7-HC) and 4-hydroxycoumarin (4-HC) in Noni (Morinda citrifolia) was described. The coumarin derivatives were determined by HPLC-UV or -fluorescence detection. More than 95% of peak purity for coumarin derivatives in Noni sample was confirmed by a multi-wavelength fluorescence detector. Amounts of scopoletin and 7-HC in Noni juices (A–H) were ranging 5.1–231 μg/ml and 0.04–0.45 μg/ml, respectively (n = 12). No 4-HC was detected in any Noni samples examined. |
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Keywords: | Noni (Morinda citrifolia) Coumarin derivatives HPLC Reactive oxygen species Antioxidative activity Chemiluminescent assay |
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