Study on chemical composition,antioxidant and anti-inflammatory activities of hot water extract from Piceamariana bark and its proanthocyanidin-rich fractions |
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| Authors: | Papa Niokhor Diouf Tatjana Stevanovic Alain Cloutier |
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| Affiliation: | 1. Centre de Recherche sur le Bois, Département des Sciences du Bois et de la Forêt, Faculté de Foresterie et Géomatique, Université Laval, QC, Canada G1K 7P4;2. Institut des Nutraceutiques et des Aliments Fonctionnels, Université Laval, QC, Canada G1K 7P4 |
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| Abstract: | Hot water extract (HWE) from Picea mariana (black spruce) bark was evaluated for its antioxidant and anti-inflammatory activities. HWE was fractionated into an oligomeric proanthocyanidin-rich fraction (OPF) and a polymeric proanthocyanidin-rich (PPF) fraction, and their bioactivities were also evaluated. OPF exhibited the highest antioxidant and anti-inflammatory activities. The compositions of HWE, OPF and PPF were also evaluated. OPF was richest in total polyphenol but the lowest in total proanthocyanidin (PA) content. Thiolysis coupled with HPLC-DAD has shown that the PAs of the black spruce bark were procyanidin type. The mean degrees of polymerisation (DPm) of HWE, OPF and PPF were, respectively, 6.0, 3.3 and 7.6. |
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| Keywords: | Picea mariana Hot water bark extractives Antioxidant activity Anti-inflammatory activity Proanthocyanidins Total polyphenol index Total proanthocyanidin content |
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