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Displacement fields using correlation methods as a tool to investigate cell migration in 3D collagen gels |
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| Authors: | ARNOLD FERTIN LAURE LAFORGUE ALAIN DUPERRAY VALÉRIE M LAURENT YVES USSON CLAUDE VERDIER |
| Affiliation: | 1. CNRS, TIMC–IMAG, University Grenoble Alpes, Grenoble, France;2. Institute for Advanced Biosciences, INSERM U 1209, CNRS UMR 5309, University Grenoble Alpes, Grenoble, France
CNRS, LIPhy, University Grenoble Alpes, Grenoble, France;3. Institute for Advanced Biosciences, INSERM U 1209, CNRS UMR 5309, University Grenoble Alpes, Grenoble, France;4. CNRS, LIPhy, University Grenoble Alpes, Grenoble, France |
| Abstract: | Living cells embedded in a complex extra-cellular matrix migrate in a sophisticated way thanks to adhesions to matrix fibres and contractility. It is important to know what kind of forces are exerted by the cells. Here, we use reflectance confocal microscopy to locate fibres accurately and determine displacement fields. Correlation techniques are used to this aim, coupled with proper digital image processing. Benchmark tests validate the method in the case of shear and stretching motions. Finally, the method is tested successfully for studying cancer cells migrating in collagen gels of different concentration. |
| Keywords: | Cancer cells cell migration confocal microscopy displacement field fibres phase correlation |